![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4482014:Design
Designed by: Mustafo Mustafokulov Group: iGEM22_NYC_Empire_State (2022-10-10)
Human insulin receptor monoclonal antibody (HIRMAb)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3501
Illegal SpeI site found at 821
Illegal PstI site found at 2910
Illegal PstI site found at 3076 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1516
Illegal BsaI.rc site found at 598
Illegal SapI.rc site found at 1864
Design Notes
The DNA sequence was codon optimized for gene synthesis and for expression in mammalian cells.
Source
This part was engineered through PCR and Gibson clonings.