Coding

Part:BBa_K4482014:Design

Designed by: Mustafo Mustafokulov   Group: iGEM22_NYC_Empire_State   (2022-10-10)


Human insulin receptor monoclonal antibody (HIRMAb)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3501
    Illegal SpeI site found at 821
    Illegal PstI site found at 2910
    Illegal PstI site found at 3076
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1516
    Illegal BsaI.rc site found at 598
    Illegal SapI.rc site found at 1864


Design Notes

The DNA sequence was codon optimized for gene synthesis and for expression in mammalian cells.


Source

This part was engineered through PCR and Gibson clonings.

References